Publications
Structural basis of HIV-1 maturation inhibitor binding and activity
Sarkar S, Zadrozny KK, Zadorozhnyi R, Russell RW, Quinn CM, Kleinpeter A, Ablan S, Meshkin H, Perilla JR, Freed EO, Ganser-Pornillos BK, Pornillos O, Gronenborn AM, Polenova T.
Nat Commun. 2023 Mar 4;14(1):1237. PMID: 36871077
A molecular switch modulates assembly and host factor binding of the HIV-1 capsid
Schirra RT, Dos Santos NFB, Zadrozny KK, Kucharska I, Ganser-Pornillos BK, Pornillos O.
Nat Struct Mol Biol. 2023 Mar;30(3):383-390. doi: 10.1038/s41594-022-00913-5. Epub 2023 Feb 9.PMID: 36759579
Purification and structure of luminal domain C of human Niemann-Pick C1 protein
Odongo L, Zadrozny KK, Diehl WE, Luban J, White JM, Ganser-Pornillos BK, Tamm LK, Pornillos O.
Acta Crystallogr F Struct Biol Commun. 2023 Feb 1;79(Pt 2):45-50. Epub 2023 Feb 2.PMID: 36748341
Strain and rupture of HIV-1 capsids during uncoating
Yu A, Lee EMY, Briggs JAG, Ganser-Pornillos BK, Pornillos O, Voth GA.
Proc Natl Acad Sci U S A. 2022 Mar 8;119(10). Epub 2022 Mar 1.PMID: 35238630
Poly(ADP-ribose) potentiates ZAP antiviral activity
Xue G, Braczyk K, Gonçalves-Carneiro D, Dawidziak DM, Sanchez K, Ong H, Wan Y, Zadrozny KK, Ganser-Pornillos BK, Bieniasz PD, Pornillos O.
PLoS Pathog. 2022 Feb 7;18(2):e1009202. eCollection 2022 Feb.PMID: 35130321
Determination of Histidine Protonation States in Proteins by Fast Magic Angle Spinning NMR
Zadorozhnyi R, Sarkar S, Quinn CM, Zadrozny KK, Ganser-Pornillos BK, Pornillos O, Gronenborn AM, Polenova T.
Front Mol Biosci. 2021 Dec 10;8:767040. eCollection 2021.PMID: 34957215
Structural evidence that MOAP1 and PEG10 are derived from retrovirus/retrotransposon Gag proteins
Zurowska K, Alam A, Ganser-Pornillos BK, Pornillos O.
Proteins. 2022 Jan;90(1):309-313. Epub 2021 Aug 21.PMID: 34357660
Structural Determinants of Virion Assembly and Release in the C Terminus of the Mason-Pfizer Monkey Virus Capsid Protein
Buckmaster MV, Zadrozny KK, Ganser-Pornillos BK, Pornillos O, Goff SP.
J Virol. 2021 Sep 9;95(19):e0061521. Epub 2021 Jul 21.PMID: 34287037
Capsid Lattice Destabilization Leads to Premature Loss of the Viral Genome and Integrase Enzyme during HIV-1 Infection
Eschbach JE, Elliott JL, Li W, Zadrozny KK, Davis K, Mohammed SJ, Lawson DQ, Pornillos O, Engelman AN, Kutluay SB.
J Virol. 2020 Dec 22;95(2):e00984-20. Print 2020 Dec 22.PMID: 33115869
Reconstitution and visualization of HIV-1 capsid-dependent replication and integration in vitro
Christensen DE, Ganser-Pornillos BK, Johnson JS, Pornillos O, Sundquist WI.
Science. 2020 Oct 9;370(6513):eabc8420. PMID: 33033190
HIV-cell membrane fusion intermediates are restricted by Serincs as revealed by cryo-electron and TIRF microscopy
Ward AE, Kiessling V, Pornillos O, White JM, Ganser-Pornillos BK, Tamm LK.
J Biol Chem. 2020 Nov 6;295(45):15183-15195. Epub 2020 Aug 11.PMID: 32788212
TRIM5α self-assembly and compartmentalization of the HIV-1 viral capsid
Yu A, Skorupka KA, Pak AJ, Ganser-Pornillos BK, Pornillos O, Voth GA.
Nat Commun. 2020 Mar 11;11(1):1307. PMID: 32161265
Hierarchical assembly governs TRIM5α recognition of HIV-1 and retroviral capsids
Skorupka KA, Roganowicz MD, Christensen DE, Wan Y, Pornillos O, Ganser-Pornillos BK.
Sci Adv. 2019 Nov 27;5(11):eaaw3631. eCollection 2019 Nov.PMID: 31807695
Biochemical Reconstitution of HIV-1 Assembly and Maturation
Kucharska I, Ding P, Zadrozny KK, Dick RA, Summers MF, Ganser-Pornillos BK, Pornillos O.
J Virol. 2020 Feb 14;94(5):e01844-19. Print 2020 Feb 14.PMID: 31801870
Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State
Kueck T, Bloyet LM, Cassella E, Zang T, Schmidt F, Brusic V, Tekes G, Pornillos O, Whelan SPJ, Bieniasz PD.
J Virol. 2019 Nov 26;93(24):e01372-19. Print 2019 Dec 15.PMID: 31578292
Restriction of HIV-1 and other retroviruses by TRIM5
Ganser-Pornillos BK, Pornillos O.
Nat Rev Microbiol. 2019 Sep;17(9):546-556. Epub 2019 Jul 16.PMID: 31312031 .Review.
Maturation of retroviruses
Pornillos O, Ganser-Pornillos BK.
Curr Opin Virol. 2019 Jun;36:47-55. Epub 2019 Jun 8.PMID: 31185449. Review.
Exploration of the TRIM Fold of MuRF1 Using EPR Reveals a Canonical Antiparallel Structure and Extended COS-Box
Stevens M, Franke B, Skorupka KA, Cafiso DS, Pornillos O, Mayans O, Norman DG.
J Mol Biol. 2019 Jul 12;431(15):2900-2909. Epub 2019 May 22.PMID: 31125568
Retrovirus Restriction by TRIM5α: RINGside at a Cage Fight
Sundquist WI, Pornillos O.
Cell Host Microbe. 2018 Dec 12;24(6):751-753. PMID: 30543772
TRIM25 Binds RNA to Modulate Cellular Anti-viral Defense
Sanchez JG, Sparrer KMJ, Chiang C, Reis RA, Chiang JJ, Zurenski MA, Wan Y, Gack MU, Pornillos O.
J Mol Biol. 2018 Dec 7;430(24):5280-5293. Epub 2018 Oct 17.PMID: 30342007
Author Correction: Inositol phosphates are assembly co-factors for HIV-1
Dick RA, Zadrozny KK, Xu C, Schur FKM, Lyddon TD, Ricana CL, Wagner JM, Perilla JR, Ganser-Pornillos BK, Johnson MC, Pornillos O, Vogt VM.
Nature. 2018 Nov;563(7731):E22. PMID: 30158708
Inositol phosphates are assembly co-factors for HIV-1
Dick RA, Zadrozny KK, Xu C, Schur FKM, Lyddon TD, Ricana CL, Wagner JM, Perilla JR, Ganser-Pornillos BK, Johnson MC, Pornillos O, Vogt VM.
Nature. 2018 Aug;560(7719):509-512. Epub 2018 Aug 1.PMID: 30069050
Nup153 Unlocks the Nuclear Pore Complex for HIV-1 Nuclear Translocation in Nondividing Cells
Buffone C, Martinez-Lopez A, Fricke T, Opp S, Severgnini M, Cifola I, Petiti L, Frabetti S, Skorupka K, Zadrozny KK, Ganser-Pornillos BK, Pornillos O, Di Nunzio F, Diaz-Griffero F.
J Virol. 2018 Sep 12;92(19):e00648-18. Print 2018 Oct 1.PMID: 29997211
General Model for Retroviral Capsid Pattern Recognition by TRIM5 Proteins
Wagner JM, Christensen DE, Bhattacharya A, Dawidziak DM, Roganowicz MD, Wan Y, Pumroy RA, Demeler B, Ivanov DN, Ganser-Pornillos BK, Sundquist WI, Pornillos O.
J Virol. 2018 Jan 30;92(4):e01563-17. Print 2018 Feb 15.PMID: 29187540
TRIM5α SPRY/coiled-coil interactions optimize avid retroviral capsid recognition
Roganowicz MD, Komurlu S, Mukherjee S, Plewka J, Alam SL, Skorupka KA, Wan Y, Dawidowski D, Cafiso DS, Ganser-Pornillos BK, Campbell EM, Pornillos O.
PLoS Pathog. 2017 Oct 17;13(10):e1006686. eCollection 2017 Oct.PMID: 29040325
TRIM23 mediates virus-induced autophagy via activation of TBK1
Sparrer KMJ, Gableske S, Zurenski MA, Parker ZM, Full F, Baumgart GJ, Kato J, Pacheco-Rodriguez G, Liang C, Pornillos O, Moss J, Vaughan M, Gack MU.
Nat Microbiol. 2017 Nov;2(11):1543-1557. Epub 2017 Sep 4.PMID: 28871090
Structure and catalytic activation of the TRIM23 RING E3 ubiquitin ligase
Dawidziak DM, Sanchez JG, Wagner JM, Ganser-Pornillos BK, Pornillos O.
Proteins. 2017 Oct;85(10):1957-1961. Epub 2017 Jul 24.PMID: 28681414
Mechanism of Ska Recruitment by Ndc80 Complexes to Kinetochores
Janczyk PŁ, Skorupka KA, Tooley JG, Matson DR, Kestner CA, West T, Pornillos O, Stukenberg PT.
Dev Cell. 2017 May 22;41(4):438-449.e4. PMID: 28535377
Mechanism of TRIM25 Catalytic Activation in the Antiviral RIG-I Pathway
Sanchez JG, Chiang JJ, Sparrer KMJ, Alam SL, Chi M, Roganowicz MD, Sankaran B, Gack MU, Pornillos O.
Cell Rep. 2016 Aug 2;16(5):1315-1325. Epub 2016 Jul 14.PMID: 27425606
Crystal structure of an HIV assembly and maturation switch
Wagner JM, Zadrozny KK, Chrustowicz J, Purdy MD, Yeager M, Ganser-Pornillos BK, Pornillos O.
Elife. 2016 Jul 14;5:e17063. PMID: 27416583
Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids
Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, Sundquist WI.
Elife. 2016 Jun 2;5:e16269. PMID: 27253068
Mechanism of B-box 2 domain-mediated higher-order assembly of the retroviral restriction factor TRIM5α
Wagner JM, Roganowicz MD, Skorupka K, Alam SL, Christensen D, Doss G, Wan Y, Frank GA, Ganser-Pornillos BK, Sundquist WI, Pornillos O.
Elife. 2016 Jun 2;5:e16309. PMID: 27253059
STRUCTURAL VIROLOGY. X-ray crystal structures of native HIV-1 capsid protein reveal conformational variability
Gres AT, Kirby KA, KewalRamani VN, Tanner JJ, Pornillos O, Sarafianos SG.
Science. 2015 Jul 3;349(6243):99-103. Epub 2015 Jun 4.PMID: 26044298
Structural basis of HIV-1 capsid recognition by PF74 and CPSF6
Bhattacharya A, Alam SL, Fricke T, Zadrozny K, Sedzicki J, Taylor AB, Demeler B, Pornillos O, Ganser-Pornillos BK, Diaz-Griffero F, Ivanov DN, Yeager M.
Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):18625-30. Epub 2014 Dec 17.PMID: 25518861
The tripartite motif coiled-coil is an elongated antiparallel hairpin dimer
Sanchez JG, Okreglicka K, Chandrasekaran V, Welker JM, Sundquist WI, Pornillos O.
Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2494-9. Epub 2014 Feb 3.PMID: 24550273
Assembly and architecture of HIV
Ganser-Pornillos BK, Yeager M, Pornillos O.
Adv Exp Med Biol. 2012;726:441-65. PMID: 22297526. Review.
Atomic-level modelling of the HIV capsid
Pornillos O, Ganser-Pornillos BK, Yeager M.
Nature. 2011 Jan 20;469(7330):424-7. PMID: 21248851
Hexagonal assembly of a restricting TRIM5alpha protein
Ganser-Pornillos BK, Chandrasekaran V, Pornillos O, Sodroski JG, Sundquist WI, Yeager M.
Proc Natl Acad Sci U S A. 2011 Jan 11;108(2):534-9. Epub 2010 Dec 27.PMID: 21187419
Disulfide bond stabilization of the hexameric capsomer of human immunodeficiency virus
Pornillos O, Ganser-Pornillos BK, Banumathi S, Hua Y, Yeager M.
J Mol Biol. 2010 Sep 3;401(5):985-95. Epub 2010 Jun 26.PMID: 20600115
Inhibition of protein kinase CK2 expression and activity blocks tumor cell growth
Zhu D, Hensel J, Hilgraf R, Abbasian M, Pornillos O, Deyanat-Yazdi G, Hua XH, Cox S.
Mol Cell Biochem. 2010 Jan;333(1-2):159-67. Epub 2009 Jul 21.PMID: 19629644
X-ray structures of the hexameric building block of the HIV capsid
Pornillos O, Ganser-Pornillos BK, Kelly BN, Hua Y, Whitby FG, Stout CD, Sundquist WI, Hill CP, Yeager M.
Cell. 2009 Jun 26;137(7):1282-92. Epub 2009 Jun 11.PMID: 19523676
X-ray structure of EmrE supports dual topology model
Chen YJ, Pornillos O, Lieu S, Ma C, Chen AP, Chang G.
Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):18999-9004. Epub 2007 Nov 16.PMID: 18024586
Functional characterization of the heterooligomeric EbrAB multidrug efflux transporter of Bacillus subtilis
Zhang Z, Ma C, Pornillos O, Xiu X, Chang G, Saier MH Jr.
Biochemistry. 2007 May 1;46(17):5218-25. Epub 2007 Apr 7.PMID: 17417881
Retraction
Chang G, Roth CB, Reyes CL, Pornillos O, Chen YJ, Chen AP.
Science. 2006 Dec 22;314(5807):1875. PMID: 17185584
Inverted repeat domains in membrane proteins
Pornillos O, Chang G.
FEBS Lett. 2006 Jan 23;580(2):358-62. Epub 2005 Dec 28.PMID: 16406365. Review.
X-ray structure of the EmrE multidrug transporter in complex with a substrate
Pornillos O, Chen YJ, Chen AP, Chang G.
Science. 2005 Dec 23;310(5756):1950-3. PMID: 16373573 Retracted.
HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein
Pornillos O, Higginson DS, Stray KM, Fisher RD, Garrus JE, Payne M, He GP, Wang HE, Morham SG, Sundquist WI.
J Cell Biol. 2003 Aug 4;162(3):425-34. PMID: 12900394
Mechanisms of enveloped RNA virus budding
Pornillos O, Garrus JE, Sundquist WI.
Trends Cell Biol. 2002 Dec;12(12):569-79. PMID: 12495845 Review.
Structure of the Tsg101 UEV domain in complex with the PTAP motif of the HIV-1 p6 protein
Pornillos O, Alam SL, Davis DR, Sundquist WI.
Nat Struct Biol. 2002 Nov;9(11):812-7. PMID: 12379843
Structure and functional interactions of the Tsg101 UEV domain
Pornillos O, Alam SL, Rich RL, Myszka DG, Davis DR, Sundquist WI.
EMBO J. 2002 May 15;21(10):2397-406. PMID: 12006492